Catalyzed by Laccase from Trametes versicolor oxidation of 7,8-dihydroxy-4-hydroxymethyl coumarin.
DOI:
https://doi.org/10.31558/2617-0876.2017.1.6Keywords:
laccase, enzymatic oxidation, mediator, coumarinAbstract
The kinetics of the reaction of laccase oxidation of 7,8-dihydroxy-4-hydroxymethylcoumarin, hydroquinone and flavonoide quercetin with molecular oxygen in an aqueous-organic medium was studied. The influence of the content of dimethylsulfoxide on the activity of laccase from Trametes versicolor in the reaction of enzymatic oxidation of phenols with molecular oxygen was established. Analysis of the kinetic data of laccase oxidation of phenolic compounds under the experimental conditions allows to conclude that 7,8-dihydroxy-4-hydroxymethylcoumarin is a more reactive phenol compared to the standard substrate of laccase hydroquinone and flavonoid quercetin. This indicates the prospectivity of studying coumarin derivatives as compounds with potentially high mediator properties.References
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